| Autor: |
Al-Taie, Oliver Hatem, Uceyler, Nurcan, Eu&szelig;ner, Ursula, Jakob, Franz, Mörk, Hubert, Scheurlen, Michael, Brigelius-Flohe, Regina, Schöttker, Katrin, Abel, Josef, Thalheimer, Andreas, Katzenberger, Tiemo, Illert, Bertram, Melcher, Ralf, Köhrle, Josef |
| Předmět: |
|
| Zdroj: |
Nutrition & Cancer; 2004, Vol. 48 Issue 1, p6-14, 9p |
| Abstrakt: |
The trace element selenium is discussed as a chemopreventive agent in colorectal carcinogenesis. Selenocysteine-containing proteins, so-called selenoproteins, represent potential molecular targets for nutritive selenium supplementation. Due to their antioxidative potential, the selenoproteins gastrointestinal glutathione peroxidase (GI-GPx) and selenoprotein P (SePP) are considered to provide protection against reactive oxygen species (ROS), thereby reducing DNA damage and preventing development of colon cancer. GI-GPx and SePP are abundantly expressed in normal colon mucosa. Recently, we demonstrated both reduced SePP expression and increased GI-GPx expression in colorectal adenomas. In this study, we investigated the expression of SePP and GI-GPx in colorectal cancers compared with corresponding normal mucosa. Further, the occurrence of genetic alterations within the SePP and GI-GPx genes was analyzed. We observed a significant reduction or loss of SePP mRNA expression in colon cancers, whereas GI-GPx mRNA and protein expression varied between different tumor samples. In addition, we identified novel polymorphisms within the SePP and GI-GPx genes with so far unknown relevance for protein function. Our results argue against a general decrease of selenoprotein expression in colorectal carcinogenesis but imply specific differential regulation of expression of individual selenoproteins. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
