| Autor: |
Shakhbazau, Antos, Danilkovich, Natalya, Seviaryn, Ihar, Ermilova, Tatyana, Kosmacheva, Svetlana |
| Zdroj: |
Molecular Biology Reports; Feb2019, Vol. 46 Issue 1, p1343-1348, 6p |
| Abstrakt: |
Radiation induces DNA and protein damage and free radical formation, effectively establishing cellular senescence in a variety of models. We demonstrate the effects of two known pleiotropic drugs following gamma radiation damage in neurosphere/cerebral organoid system based on human embryonic stem cells. mTORC1 repression by rapamycin prior to irradiation, or metabolic activation by minocycline after irradiation, partially rescues neuroepithelium integrity, neurite-growing capacity, ventricle formation and extracellular acidification rate as an integral measure of metabolic output. Cerebral organoid model thus provides valid and robust readouts for radiation studies in a complex 3D setting. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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