Phenotypic and Molecular Study of mecA Gene in MRSA Isolated from Clinical Cases in Misan Province/Iraq.

Autor: Aziz, Zahid S., Hassan, Marwa A.
Předmět:
Zdroj: Indian Journal of Public Health Research & Development; Feb2019, Vol. 10 Issue 2, p553-558, 6p
Abstrakt: Methicillin-Resistant Staphylococcus aureus (MRSA) is a common strain of S. aureus caused infections lead to increased of risk in clinical outcomes and death, and compared with infections caused by non-resistant strains of the same bacteria patients consume more resources of health-care, where their hospitalization was prolonged because most preferred drugs such as β-lactam antibiotics not have the ability to produce bactericidal effect on this bacteria. Therefore this study aimed to: Isolation and identification of MRSA by using conventional and confirmatory techniques, detection of phenotypic traits related to their antibiotic resistance and estimation the occurrence of mecA gene in identified bacteria. A total of 274 samples, were collected from local hospitals in Misan Province during the period from December 2017 to April 2018. Diagnostic results using microscopic, cultural, biochemical tests and vitek 2 system proved that 106 bacterial isolates belong to Staphylococcus aureus. The susceptibility test of S. aureus isolates was performed toward many types of antibiotics, from all isolates 99(93.4%) were determined as MRSA by oxacillin antibiotic and 93(87.7%) were determined as MRSA by cefoxitin antibiotic. The results of molecular estimation of mecA gene showed that 89(89.9%) and 91(97.8%) from phenotypically positive MRSA isolates had this gene for oxacillin and cefoxitin antibiotics respectively. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index