Methodological Features of the Morphometric Characterization of the Synaptoarchitectonics of the Human Neocortex by Immunofluorescent Detection of Neuromodulin.

Autor: Stepanov, A. S., Akulinin, V. A., Stepanov, S. S., Avdeev, D. B.
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Zdroj: Neuroscience & Behavioral Physiology; Jan2019, Vol. 49 Issue 1, p103-108, 6p
Abstrakt: Objective. To study the possibility of using GAP-43 for morphometric characterization of synaptoarchitectonics. Materials and methods. Studies used immunofluorescent detection of neuromodulin (GAP-43) by confocal microscopy and automated computer image analysis of layer V (field 4) of the human brain (n = 4) using ImageJ 1.46. Results. Immunofluorescent detection of GAP-43 identified the distribution of axodendritic and axosomatic synapses, the total area of axon terminals, and the number densities of intermediate and large terminals. Conclusions. A necessary condition for obtaining accurate data is having digital images with sufficient resolution (600-900 pixels per inch). These results need to be considered in studies of the synaptoarchitectonics of the neocortex using immunohistochemical methods for investigating synapse structure. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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