| Autor: |
Gong, Zhen-Hua, Zhou, Feng, Shi, Chao, Xiang, Tie, Zhou, Chang-Kai, Wang, Qian-Qian, Jiang, Ya-Su, Gao, Sheng-Feng |
| Zdroj: |
Cellular & Molecular Biology Letters; 3/8/2019, Vol. 24 Issue 1, pN.PAG-N.PAG, 1p |
| Abstrakt: |
Background: Cutaneous squamous cell carcinoma (CSCC) is a common type of skin malignancy. MicroRNA-221 (miRNA-221) is a critical non-coding RNA in tumor initiation and progression. However, the molecular mechanisms of miRNA-221 in the development of CSCC remain unknown. This study investigated the expression of miRNA-221 in CSCC and its potential tumor biological functions. Methods: MTT assay, colony assay, PCR, and Western blot were adopted. Results: In this study, miRNA-221 expression was significantly higher in CSCC tissues and cell lines than in normal tissues and cells (P < 0.05). Further functional experiments indicated that miRNA-221 knockdown inhibited the proliferation and cell cycle, while upregulation of miRNA-221 presented the opposite role. The dual reporter gene assays indicated that PTEN is a direct target gene of miRNA-221. PTEN protein or mRNA levels were decreased after the cells were transfected with miR-221 mimics. Conclusions: Taken together, the obtained results indicated that miR-221 plays an oncogenic function in CSCC by targeting PTEN and further suggest that miR-221 may be a potential target for CSCC diagnosis and treatment. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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