Positive Correlation between Activated CypA/CD147 Signaling and MMP-9 Expression in Mice Inflammatory Periapical Lesion.
Autor: | Wang, Yan-qing, Zhang, Jie, Zhu, Ling-xin, Yu, Jing-jing, Liu, Ming-wen, Zhu, Shen-ting, Liu, Guo-jing, Peng, Bin |
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Předmět: |
ANIMAL experimentation
BONE resorption BONE growth CELL receptors CELLULAR signal transduction COMPUTED tomography CYTOKINES FLUORESCENT antibody technique GENE expression HISTOLOGICAL techniques IMMUNOHISTOCHEMISTRY INFLAMMATION MACROPHAGES MANDIBLE MICE MOLARS PERIAPICAL diseases WESTERN immunoblotting X-rays DISEASE progression LIPOPOLYSACCHARIDES MATRIX metalloproteinases |
Zdroj: | BioMed Research International; 3/5/2019, p1-10, 10p |
Abstrakt: | Aim. Cyclophilin A (CypA)/CD147 signaling plays critical roles in the regulation of inflammation and bone metabolism. This study aimed to investigate the participation of CypA/CD147 in mice periapical lesions progression and its relationship with bone resorption. Methodology. Periapical lesions were induced by pulp exposure in the first lower molars of 40 C57BL/6J mice. The mice were sacrificed on days 0, 7, 14, 21, 28, 35, 42, and 49. Mandibles were harvested for X-ray imaging, microcomputed tomography scanning, histologic observation, immunohistochemistry, enzyme histochemistry, and double immunofluorescence analysis. Western blot was employed to further detect the related molecular signaling pathways in LPS-stimulated RAW 264.7 cells treated with CypA inhibitor. Results. The volume and area of the periapical lesions increased from day 0 to day 35 and remained comparably stable until day 49. Immunohistochemistry demonstrated that the CypA expression levels also increased from day 0 to day 35 and decreased until day 49, similar to CD147 expression (R2 = 0.4423, P < 0.05), osteoclast number (R2 = 0.5101, P < 0.01), and the expression of osteoclastogenesis-related matrix metalloproteinase 9 (MMP-9) (R2 = 0.4715, P < 0.05). Serial sections further confirmed the colocalization of CypA and CD147 on osteoclasts with immunohistochemistry. And the distribution of CypA-positive or CD147-positive cells was positively correlated with the dynamics of MMP-9-positive cells by using immunofluorescence analysis. Furthermore, CD147 and MMP-9 expression in RAW 264.7 cells were both downregulated with CypA inhibitor treatment (P < 0.05). Conclusions. The present study reveals the positive correlation of CypA/CD147 signaling and osteoclast-related MMP-9 expression in mice inflammatory periapical lesions progression. Therefore, intervention of CypA/CD147 signaling could probably provide a potential therapeutic target for attenuating inflammatory bone resorption. [ABSTRACT FROM AUTHOR] |
Databáze: | Complementary Index |
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