Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway.
Autor: | Zhao, Zhengxiao, Liu, Baojun, Sun, Jing, Lu, Linwei, Liu, Lumei, Qiu, Jian, Li, Qiuping, Yan, Chen, Jiang, Shan, Mohammadtursun, Nabijan, Ma, Wenjuan, Li, Mihui, Dong, Jingcheng, Gong, Weiyi |
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Předmět: |
ANIMAL experimentation
BIOMARKERS BIOCHEMISTRY BIOLOGICAL models CANCER patients CELL differentiation CELL lines CELLULAR signal transduction COMPUTED tomography CYTOSKELETAL proteins DRUG delivery systems EPITHELIAL cells GENE expression GLYCOPROTEINS LUNGS LUNG cancer PHENOMENOLOGY MICE MOLECULAR structure PHOSPHORYLATION PROTEIN kinases STEM cells TRANSCRIPTION factors WESTERN immunoblotting XENOGRAFTS VASCULAR endothelial growth factors FLAVONES IN vitro studies IN vivo studies |
Zdroj: | Evidence-based Complementary & Alternative Medicine (eCAM); 3/4/2019, p1-7, 7p, 2 Diagrams, 3 Graphs |
Abstrakt: | Non-small cell lung cancer (NSCLC) is one of the most lethal cancers worldwide. Inhibitor of differentiation 1 (Id1) is the member mostly linked to tumorigenesis in Id family and a potential molecular target in cancer therapy. In the current study, we established an orthotopic lung cancer model by injecting athymic nude mice with A549 cells and evaluated the antitumor effect of baicalein and expression of Id1-related proteins in vivo and in vitro. Micro-CT images showed that tumor volume in baicalein group was significantly reduced. Western blot analysis revealed that baicalein suppressed the expression of Id1 protein, epithelial-to-mesenchymal transition (EMT) related molecules (N-Cadherin, vimentin), and angiogenesis related protein (VEGF-A), accompanied by upregulation of epithelial markers (such as E-cadherin). In addition, phosphorylation of upstream molecular Src was significantly restrained after baicalein treatment. This study firstly demonstrates that baicalein inhibits tumor growth in orthotopic human NSCLC xenografts via targeting Src/Id1 pathway. [ABSTRACT FROM AUTHOR] |
Databáze: | Complementary Index |
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