| Autor: |
Abdel-Hay, Nesma Hussein, Ghalia, Azza Hassan Abou, Mohammed, Dalia Abdel-Wahab, Basheer, Ayman Ragaa, Seif, Ansam Aly |
| Předmět: |
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| Zdroj: |
Journal of Stem Cells; 2018, Vol. 13 Issue 3, p127-137, 11p |
| Abstrakt: |
Background: Stem cells created new hope in regenerative medicine for their ability to repair damaged tissues. This present study examined the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on miRNA-133a expression, and their relation to cardiac complications in diabetic rats. Methods: The current study included three groups of Wistar rats: control rats, type 1 diabetes rat model induced by streptozotocin (STZ), and diabetic rats treated with BMMSCs. For all rats, fasting blood glucose (FBG), serum insulin as well as cardiovascular functions (heart rate, systolic blood pressure and electrocardiogram) were assessed. All rats were sacrificed after eight weeks. Specimens from cardiac and pancreatic tissues were examined to detect fluorescent labelled BM-MSCs, and their regenerative effect by hematoxylin and eosin stain. Expression of miRNA-133a was assessed in pancreatic and cardiac tissues by quantitative real time polymerase chain reaction (qRT-PCR). Results: This study revealed that the body weight and serum insulin levels were increased in BM-MSC treated diabetic rats, while FBG didn't reach the euglycemic level. Moreover, improvement of systolic blood pressure and corrected QT (QTc) interval was found. The expression of miR-133a was significantly increased in pancreatic and cardiac tissues of diabetic rats, and correlated with FBG. However, there was no significant difference in its expression after treatment with BM-MSCs. Conclusion: This study has given evidence for the first time that miR133a was expressed in pancreatic as well as cardiac tissues of diabetic rats in response to hyperglycemia. Also, BM-MSCs had the potential to improve pancreatic and cardiac functions in diabetic rats. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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