Synthesis of a New Vitamin D3 Hapten and Its Protein Conjugates.

Autor:	Litvinovskaya, R. P., Savachka, A. P., Sauchuk, A. L., Pradko, A. G., Mirantsova, T. V., Khripach, V. A.
Předmět:	VITAMIN D HAPTENS PROTEIN synthesis BIOCONJUGATES SERUM albumin
Zdroj:	Russian Journal of Bioorganic Chemistry; Nov2018, Vol. 44 Issue 6, p778-786, 9p
Abstrakt:	The synthesis of 25-(1-carboxymethoxy)-imino-3(S)-hydroxy-9,10-seco-27-norcholesta-5(Z),7(E),10(19)-triene, a new hapten of vitamin D3, has been carried out in ten steps from ergosterol. The hapten was conjugated to horseradish peroxidase and bovine serum albumin. Antibodies to 25-hydroxyvitamin D3 were obtained using the synthesized conjugates. An immunochemical system was developed for the quantitative determination of 25-hydroxyvitamin D3. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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