| Autor: |
Takacs, Ferenc Zoltan, Radosa, Julia Caroline, Bohle, Rainer Maria, Bochen, Florian, Juhasz-Böss, Ingolf, Solomayer, Erich-Franz, Schick, Bernard, Linxweiler, Maximilian |
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| Zdroj: |
Archives of Gynecology & Obstetrics; Feb2019, Vol. 299 Issue 2, p481-488, 8p, 1 Diagram, 2 Charts, 1 Graph |
| Abstrakt: |
Purpose: In the previous studies, we demonstrated that Sec62 is essential for tumor cell migration, epithelial-to-mesenchymal transition, and intracellular stress tolerance. An increase in Sec62 expression correlated with an increase in cervical dysplasia severity in liquid-based cytology specimens. Ki67 is an established proliferation marker. Thus, in this study, we examined a method of Sec62/Ki67 dual staining for the detection of high-grade dysplasia and cancer in cervical liquid-based cytology specimens.Methods: Sec62/Ki67 dual staining was performed on 100 cervical liquid-based cytology specimens. The staining results were correlated with cytological, immunocytological (p16/Ki67), colposcopic, and histological findings.Results: All 56 (n = 56, 100%) cases of cervical intraepithelial neoplasia grade 3 and cervical cancer (CIN3+ lesions) were positive for Sec62/Ki67 staining, while low-grade lesions and normal cells were negative. Sec62/Ki67 staining was highly sensitive and specific for the detection of CIN2+ and CIN3+ lesions (94.37%; 100% and 100%; 84.09%, respectively).Conclusions: Sec62/Ki67 dual-staining immunocytochemistry is a promising cytological tool for interpreting high-grade squamous lesions in cytological specimens and for assessing the risk of progression to cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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