member of the TERMINAL FLOWER 1/CENTRORADIALIS gene family controls sprout growth in potato tubers.

Autor: Morris, Wayne L, Alamar, M Carmen, Lopez-Cobollo, Rosa M, Cañete, Javier Castillo, Bennett, Mark, Kaay, Jeroen Van der, Stevens, Jennifer, Sharma, Sanjeev Kumar, McLean, Karen, Thompson, Andrew J, Terry, Leon A, Turnbull, Colin G N, Bryan, Glenn J, Taylor, Mark A
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Zdroj: Journal of Experimental Botany; 1/30/2019, Vol. 70 Issue 3, p835-843, 9p
Abstrakt: Potato tuber bud dormancy break followed by premature sprouting is a major commercial problem which results in quality losses and decreased tuber marketability. An approach to controlling premature tuber sprouting is to develop potato cultivars with a longer dormancy period and/or reduced rate of sprout growth. Our recent studies using a potato diploid population have identified several quantitative trait loci (QTLs) that are associated with tuber sprout growth. In the current study, we aim to characterize a candidate gene associated with one of the largest effect QTLs for rapid tuber sprout growth on potato chromosome 3. Underlying this QTL is a gene encoding a TERMINAL FLOWER 1/CENTRORADIALIS homologue (PGSC0003DMG400014322). Here, we use a transgenic approach to manipulate the expression level of the CEN family member in a potato tetraploid genotype (cv. Désirée). We demonstrate a clear effect of manipulation of StCEN expression, with decreased expression levels associated with an increased rate of sprout growth, and overexpressing lines showing a lower rate of sprout growth than controls. Associated with different levels of StCEN expression were different levels of abscisic acid and cytokinins, implying a role in controlling the levels of plant growth regulators in the apical meristem. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index