Floral morphogenesis in thin-layer tissue cultures of Nicotiana tabacum.

Autor: Van Den Ende, G., Croes, A. F., Kemp, A., Barendse, C. W. M., Kroh, M.
Předmět:
Zdroj: Physiologia Plantarum; Sep84, Vol. 62 Issue 1, p83-88, 6p
Abstrakt: The morphological changes in thin-layer tissues of Nicotiana tabacum L. cv. Samsun, cultured on Murashige and Skoog medium with 1 μM each of naphthalene acetic acid (NAA) and benzyladenine (BA), were studied during the first 8 days of culture with light and scanning electron microscopy. The first three days of culture are characterized by enlargement of all cells and cell divisions starting in the cortical parenchyma cells adjacent to the medium. Between days 3 and 6. epidermal and/or subepidermal cells start to divide, resulting in division centers, which lead to flower bud formation. The hormones NAA and BA in different concentrations affect the formation and distribution of flower buds, bud morphology and callus formation. BA influences particularly bud formation and bud morphology, while NAA affects callus formation in particular. In addition, polarity may occur in the formation of both callus and flower buds, the degree of which depends upon the hormone concentrations. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index