| Autor: |
Branco, Renato Chaves Souto, Camargo, Rafael Ludemann, Batista, Thiago Martins, Vettorazzi, Jean Franciesco, Lubaczeuski, Camila, Bomfim, Lucas Henrique Montes, Silveira, Leonardo Reis, Boschero, Antônio Carlos, Zoppi, Cláudio Cesar, Carneiro, Everardo Magalhães |
| Předmět: |
|
| Zdroj: |
Journal of Cellular Physiology; May2019, Vol. 234 Issue 5, p6313-6323, 11p |
| Abstrakt: |
Nutrient malnutrition, during the early stages of development, may facilitate the onset of metabolic diseases later in life. However, the consequences of nutritional insults, such as a high‐fat diet (HFD) after protein restriction, are still controversial. We assessed overall glucose homeostasis and molecular markers of mitochondrial function in the gastrocnemius muscle of protein‐restricted mice fed an HFD until early adulthood. Male C57BL/6 mice were fed a control (14% protein‐control diet) or a protein‐restricted (6% protein‐restricted diet) diet for 6 weeks. Afterward, mice received an HFD or not for 8 weeks (mice fed a control diet and HFD [CH] and mice fed a protein‐restricted diet and HFD [RH]). RH mice showed lower weight gain and fat accumulation and did not show an increase in fasting plasma glucose and insulin levels compared with CH mice. RH mice showed higher energy expenditure, increased citrate synthase, peroxisome‐proliferator‐activated receptor gamma coactivator 1‐alpha protein content, and higher levels of malate and α‐ketoglutarate compared with CH mice. Moreover, RH mice showed increased AMPc‐dependent kinase and acetyl coenzyme‐A (CoA) carboxylase phosphorylation, lower intramuscular triacylglycerol content, and similar malonyl‐CoA levels. In conclusion, protein undernourishment after weaning does not potentiate fat accumulation and insulin resistance in adult young mice fed an HFD. This outcome seems to be associated with increased skeletal muscle mitochondrial oxidative capacity and reduced lipids accumulation. Evidence suggests that overfeeding after nutrient deprivation may favor the disruption of metabolism and, therefore, favor the development of obesity, type 2 diabetes, and comorbidities. In animal models, the effects of fat‐enriched diets in protein‐restricted mice are still controversial. Protein restriction programmed skeletal muscle metabolism increased mitochondrial function and prevented fat accumulation and increment on fasting plasma glucose and insulin concentration. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text