| Autor: |
Sun, Ya-wei, Liu, Ying-ying, Wu, Hua, Wang, Ling-fei, Liu, Jian-hua, Yuan, Li, Pan, Yu-shan, He, Dan-dan, Hu, Gong-zheng |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology; 1/15/2019, pN.PAG-N.PAG, 9p |
| Abstrakt: |
Tet(M)-type proteins confer resistance to tetracycline and related antibiotics by interacting with the ribosome. Genes encoding Tet(M) have been found in a range of bacteria, including Escherichia coli. In the current study, conjugation experiments were performed between seven different tetracycline-resistant, azide-susceptible E. coli strains isolated from ducks and tetracycline-sensitive, azide-resistant E.coli J53. Transconjugants were obtained from two of the strains at a frequency of 1.2 × 10−8. PCR, southern blotting and sequencing demonstrated that tet (M) in the transconjugants was located on a ~50 kb IncHI2-type plasmid and was part of a composite transposon, designated Tn6539. This transposon is flanked by two IS26 elements in opposite orientation and contains the Tn3Δ tnpA +Δ orf13 - lp - tet (M)+gamma delta+ tnpX +Δ tnpR sequences. The Δ orf13 - lp - tet (M) sequence was a highly conserved genetic fragment in E. coli harboring tet (M) and mainly located in the composite transposons flanked by IS6-family elements. In summary, Tn6539 is a new composite transposon capable of horizontal transfer of tet (M) among E. coli isolates. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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