| Autor: |
Suzawa, T., Hyodo, S., Kishi, T., Kashewa, H., Kittaka, E., Sakano, T., Usui, T. |
| Předmět: |
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| Zdroj: |
Immunology; Dec83, Vol. 50 Issue 4, p575-580, 6p |
| Abstrakt: |
Colony growth of human lymphocytes by phorbol myristate acetate (PMA) was studied. PMA was able to induce lymphocyte colony growth in methylcellulose semi-solid cultures in the absence of lectin mitogens, phytohaemagglutinin or concanavalin A. PMA-induced colonies were found in cultures of mononuclear cells, monocyte-depleted mononuclear cells and the T-enriched cell fraction, whereas no colonies were obtained from the non-T cell fraction. At least one million mononuclear cells were required to form colonies by PMA. The colony cells were mainly I cells as judged from sheep red blood cell rosette formation. Surface immunoglobulin positive cells and peroxidase positive cells were not detected in colony cells. Non-specific esterase positive cells were only found to be less than 1% of colony cells. T cells formed more colonies than did mononuclear cells, presumably because of a concentration of colony forming cells and/or co-operating cells. Colony formation by PMA was induced from monocyte-depleted mononuclear cells and monocyte-depleted I cells, suggesting independence of monocytes. When mononuclear cells were precultured at 37° for 5 mm or 30 mm with PMA and then cultured in the semi-solid medium without PMA, only a small number of colonies grew. Further studies of PMA- induced T-cell colonies will provide information the identification and characterization of immunological states in various immunological diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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