| Autor: |
Hardard'Ottir, I., Whelan, J., Kinsella, J. E. |
| Předmět: |
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| Zdroj: |
Immunology; Aug92, Vol. 76 Issue 4, p572-577, 6p |
| Abstrakt: |
Cell-associated and secreted tumor necrosis factor (TNF), prostaglandin (PG) E2, and 6-keto PGF1α were monitored at various times following in vitro stimulation of resident peritoneal macrophages with lipopolysaccharide (LPS). Macrophages were obtained from mice maintained on diets containing 1.5 wt% n--3 polyunsaturated fatty acids (PUFA)+ 1.5 wt% n--6 fatty acids; 1.5 wt% n--6 fatty acids; or 3 wt% n--6 fatty acids, for 4 weeks. Cell-associated TNF increased transiently in the resident peritoneal macrophages from mice consuming all diets and decreased after TNF secretion had reached maximum and plateaued. Macrophages from mice consuming the n--3 PUFA contained more cell-associated TNF and secreted more TNF than macrophages from mice consuming diets containing n--6 fatty acids only, at all time-points studied. Macrophages from mice consuming the n-- 3 PUFA showed an earlier increase in cell-associated and secreted TNF compared with macrophages from mice consuming n--6 fatty acids only. Kinetics of maximum TNF production was not affected by the diets and dietary n--3 PUFA did not cause a prolonged increase in TNF secretion. Macrophages from mice consuming the n--3 PUFA produced less PG than macrophages from mice consuming the n--6 fatty acids only. PG secretion increased following appearance of cell-associated TNF but when PG had accumulated in the medium there was no further increase in TNF production. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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