| Autor: |
Leeuwenberg, J. F. M., Smeets, E. F., Neefjes, J. J., Shaffer, M. A., Cinek, T., Jeunhomme, T. M. A. A., Ahern, T. J., Buurman, W. A. |
| Předmět: |
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| Zdroj: |
Immunology; Dec92, Vol. 77 Issue 4, p543-549, 7p |
| Abstrakt: |
Endothelial cells respond to several cytokines by a rapid increase in expression of the adhesion molecules E-selectin and intercellular adhesion molecule-l (ICAM-l), followed by a gradual decline. The fate of these molecules, which was so far unknown, was studied. Specific sandwich ELISA for the detection of soluble (s)E-selectin and sICAM-l were developed. In supernatant, centrifuged 3 hr at 100,000 g to remove microparticles, from human umbilical vein endothelial cells (HUVEC) activated with tumour necrosis factor (TNF), interleukin-I (lL-l) or lipopolysaccharide (LPS), E-selectin and ICAM- I molecules could be detected. Biochemical analysis revealed that sE-selectin migrated as a band of approximately 94,000 MW. The amount of soluble adhesion molecules released was directly correlated with cell surface expression. Maximal release of E-selectin was observed 6-12 hr after activation of HUVEC and decreased to below detection limit 24 hr after activation. After activation, release of ICAM- I gradually increased with ICAM- I cell surface expression, and reached a plateau after 24 hr. which was constant for 3 days. Since E-selectin and ICAM-l are highly expressed at inflammatory sites, the resulting high concentrations of released E-selectin and ICA M- I may affect interactions of leucocytes with endothelial cells. The physiological role, however, of the release of E- selectin and ICAM-l remains to be elucidated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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