| Autor: |
Zhang, Y., Frutiger, S., Hughes, G.J., Savoy, M.-C., Barkas, T. |
| Předmět: |
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| Zdroj: |
Immunology; Dec90, Vol. 71 Issue 4, p538-543, 6p |
| Abstrakt: |
In the Lewis rat, T-cell lines from animals immunized with native or denatured Torpedo nAChR recognize the Torpedo-derived recombinant protein TαX1Ω (α-2-200), but not the equivalent mouseor chick-derived recombinant proteins X4Ω or CαX1Ω (α6-216 and α35-216, respectively). T-cell lines derived from animals immunized with TαX1Ω, X4Ω or CαX1Ω fi are specific for the homologous protein. This lack of cross-species reactivity suggests caution in the use of Torpedo nAChR-selected lines generated from human patients. Proteolysis and fractionation of the products by reverse-phase HPLC was effective in localization of a T-cell epitope of X4Ω a mouse-derived recombinant protein. With Lewis rats, the major epitope of TαX1Ω is α97-112. However, the major epitope of the mousederived protein, X4Ω, as determined by proteolytic digestion and fractionation of the products by reverse-phase HPLC, is α14-22. This shift in T-cell epitope between closely related proteins may result from the conservation of sequence of α7-112 between mammalian species. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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