Abstrakt: |
This study evaluates the Citrus sudachi Hort. ex Shirai (sudachi) peel grown in Japan and Korea as an antioxidant, an antimicrobial, and α-glucosidase inhibitory agent. In this investigation, 80% methanol (MeOH) partitioned into four different fractions-n-hexane, ethyl acetate (EtOAc), n-butanol, and aqueous, were used as solvents for sudachi. It was found that the n-butanol fraction was the highest among the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assays for both countries. For the reducing power assays, the EtOAc fraction showed the highest reducing power for both countries. The highest phenol and flavonoid content was found in the EtOAc fraction in the samples from both Korea and Japan. For the α-glucosidase inhibitory activity, Japanese sudachi demonstrated greater inhibitory activity than Korean sudachi. For the minimum inhibitory concentration (MIC) assay, gram-positive bacteria were more sensitive than gram-negative bacteria, and the EtOAc fraction showed greater inhibitory activity in samples from both countries; however, Korean sudachi exhibited the greatest inhibitory activity. The combined data from all assays indicated that sudachi can be used effectively as a natural antioxidant, antimicrobial, and α-glucosidase inhibitory agent. [ABSTRACT FROM AUTHOR] |