Glutaraldehyde as a Coupling Reagent in Passive Haemagglutination.

Autor: Onkelinx, E., Meuldermans, W., Joniau, M., Lontie, R.
Předmět:
Zdroj: Immunology; Jan69, Vol. 16 Issue 1, p35-43, 9p
Abstrakt: A sensitive passive haemagglutination technique is described depending on the attachment of proteins to erythrocytes with glutaraldehyde. Various factors that play a role in the coupling of bovine serum albumin to sheep erythrocytes are examined. A standard procedure was deduced by which other proteins were coupled and tested with their specific antisera. The observed titres are comparable to those obtained with the classical bis-diazotized benzidine method. The technique is specific and practicable. It provides sensitized cells that remain stable for several weeks.
Databáze: Complementary Index