| Autor: |
Schreiner, M., Bäumler, W., Eckl, D.B., Späth, A., König, B., Eichner, A. |
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| Zdroj: |
British Journal of Dermatology; Dec2018, Vol. 179 Issue 6, p1358-1367, 10p |
| Abstrakt: |
Summary: Background: To prevent infections that arise from the skin surface it is necessary to decolonize human skin prior to any proposed treatment or surgical intervention. Photodynamic inactivation of bacteria (PIB) uses cationic photosensitizers that attach to the surface of bacteria, generate reactive oxygen species on light irradiation and thereby kill bacteria via oxidative mechanisms. Objectives: To evaluate the potential and the safety of PIB for decolonization of bacteria from skin. Methods: PIB with the new photosensitizer SAPYR [2‐((4‐pyridinyl)methyl)‐1H‐phenalen‐1‐one chloride] was initially tested against different bacterial species in vitro. Then, ex vivo porcine skin samples were used as a model for decolonization of different bacteria species. The numbers of viable bacteria were quantified and the mitochondrial activity of skin cells was histologically analysed (using nitroblue tetrazolium chloride, NBTC). The same procedure was performed for human skin and meticillin‐resistant Staphylococcus aureus (MRSA). Results: The in vitro studies showed a 5 log10 reduction of all tested bacterial species. On ex vivo porcine skin samples, PIB reduced the viability of all tested bacterial species by at least 3 log10 steps. On human skin samples ex vivo, PIB reduced the number of viable MRSA by maximal 4·4 log10 steps (1000 μmol L−1SAPYR, incubation time 10 min, 60 J cm−2). NBTC staining showed normal mitochondrial activity in skin cells after all PIB modalities. Conclusions: The results of this study show that PIB can effectively and safely kill bacteria like MRSA on the skin surface and might have the potential of skin decolonization in vivo. What's already known about this topic? Colonization of human skin with healthcare‐associated pathogens is associated with increased risk of infection.Topical antibiotics and biocides are used to prevent bacterial skin infections.Widespread and indiscriminate use of topical agents has led to the emergence of bacterial resistance.Photodynamic inactivation of bacteria (PIB) shows excellent inactivation of all bacteria types while avoiding resistance. What does this study add? The study shows for the first time an antimicrobial effect (> 3 log10 orders) against multidrug‐resistant bacteria like meticillin‐resistant Staphylococcus aureus on ex vivo human skin samples.Prior to PIB treatment the bacteria was touch‐dry to the skin surface to ensure a realistic condition for skin decolonization.Nitroblue tetrazolium chloride staining showed no cellular damage of the skin while the bacteria were effectively inactivated. Plain language summary available online Respond to this article [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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