| Autor: |
Sancéau, J., Falcoff, R., Beranger, F., Carter, D. B., Wietzerbin, J. |
| Předmět: |
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| Zdroj: |
Immunology; Jan1990, Vol. 69 Issue 1, p52-56, 5p |
| Abstrakt: |
We studied IL-6 gene expression in human monocytes stimulated by muramyl dipeptide (MDP), a synthetic immunomodulator derived from mycobacterial cell walls. In control monocytes, two IL-6 transcripts of 3-4 kb and U6 kb were easily detected at 2-5 hr of culture and remained stable until 18 hr. In MDP-treated monocytes, three IL-6 RNA species displayed different kinetics of accumulation: a 3.4 kb RNA whose expression already reached its maximum after 2.5 hr exposure to MDP; a 1.6 kb RNA whose expression peaked at 5 hr; and a new RNA species of 1.4 kb which was transiently induced in early time of cell stimulation. TNF-α co-operated with MDP to increase IL-6 gene expression and secretion of biological active protein (measured by the hybridoma plasmacytoma growth factor assay). MDP exhibits a broad spectrum of immunomodulation properties such as adjuvant activity, enhancement of macrophage cytotoxicity against tumour and induction of non- specific resistance to intracellular agents. The results reported here suggest that these properties might be linked to the stimulation by M DP of genes coding for key cytokines such as IL-6, TNF and IL- I. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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