| Autor: |
MALL, VIJAYA SHRI, OJHA, RAJENDRA PRASAD, TIWARI, RAKESH KUMAR |
| Předmět: |
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| Zdroj: |
Oriental Journal of Chemistry; 2018, Vol. 34 Issue 5, p2440-2446, 7p |
| Abstrakt: |
Building of high affinity triplex-forming oligonucleotides(TFOs) enhances its therapeutical application. Peptide nucleic acid(PNA), a modified DNA oligomer with neutral backbone enhances the affinity of TFO. MD simulation method is very helpful to study the stability, affinity and behavior of complex at nanosecond scale. Therefore a 15-mer PNA-TFO is used here to model DNA:DNA:PNA triplex in mixed purine/pyrimidine sequence. DNA:DNA:DNA triplex, DNA:DNA duplex and DNA:PNA duplex were also modeled for comparison. 100ns of MD run on all four complexes in solution at neutral pH. The triplex conformation stabilized with Recombinant type(R-type) of Hydrogen bonding during simulation. The rmsd of DNA:DNA:PNA triplex and DNA:DNA:DNA triplex converges after 45 ns of dynamics and the binding affinity of PNA-TFO found greater than DNA-TFO. Together with non-toxicity of PNA oligomer, stable triplex formation with R-type of hydrogen bonding pattern and high binding affinity in mixed sequence promotes the study regarding Recombinant triplex with PNA-TFO. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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