| Autor: |
Mamidipudi, Vidya, Chang, Betty Y., Harte, Rachel A., Lee, Kelly C., Cartwright, Christine A. |
| Předmět: |
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| Zdroj: |
FEBS Letters; Jun2004, Vol. 567 Issue 2/3, p321-326, 6p |
| Abstrakt: |
Cancer cells are capable of serum- and anchorage-independent growth, and focus formation on monolayers of normal cells. Previously, we showed that RACK1 inhibits c-Src kinase activity and NIH3T3 cell growth. Here, we show that RACK1 partially inhibits v-Src kinase activity, and the serum- and anchorage-independent growth of v-Src transformed cells, but has no effect on focus formation. RACK1-overexpressing v-Src cells show disassembly of podosomes, which are actin-rich structures that are distinctive to fully transformed cells. Together, our results demonstrate that RACK1 overexpression in v-Src cells partially reverses the transformed phenotype of the cells. Our results identify an endogenous inhibitor of the oncogenic Src tyrosine kinase and of cell transformation. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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