| Autor: |
Srikumaran, S., Onisk, D. V., Borca, M. V., Nataraj, C., Zamb, T. J. |
| Předmět: |
|
| Zdroj: |
Immunology; Jul90, Vol. 70 Issue 3, p284-289, 6p |
| Abstrakt: |
A neutralizing murine monoclonal antibody (mAb) of the IgG2a isotype (MM-113), specific for bovine herpesvinis 1 (BHV-1) glycoprotein gIV, was used to develop anti-idiotypic antibodies (anti-Id) in a calf. The bovine anti-Id were isolated from the serum of the immunized call by affinity chromatography on an MM-113 Sepharose column, followed by repeated adsorption on a murine lgG2a column. The anti-Id thus obtained specifically reacted with MM-113, but not with isotype-matched controls. They also inhibited the binding of MM-113 to BHV-1 in a concentration- dependent manner. Mice immunized with the anti-Id produced neutralizing antibodies to BHV-1. The anti-Id bound to cells permissive to BHV-1 in a cell-binding radioimmunoassay (RIA). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
