| Autor: |
Mahantesh, Sujata, H. N., Ramesh Babu, Ghanti, Kirankumar, Prasad, T. G., Reddy, Malireddy K., Raddy, P. C. |
| Předmět: |
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| Zdroj: |
Indian Journal of Agricultural Research; Oct2018, Vol. 52 Issue 5, p489-496, 8p |
| Abstrakt: |
RNA helicases function as molecular motors that rearrange RNA secondary structure, potentially performing roles in any cellular process involving RNA metabolism in an ATP-dependent manner and play an important role in protein synthesis. Pennisetum glaucum 47 (PG47) RNA helicase overexpressed in groundnut (Arachis hypogaea L.) cultivar GPBD-4 improved drought tolerance. The transgenics plants were confirmed for presence, expression and stable integration by Kanamycin screening, genomic DNA PCR, RT-PCR and Southern analyses respectively. In T3 generation, the promising transgenic events were identified based on stress tolerance and improvement in productivity. The transgenic events showed enhanced stay-green phenotype and increased chlorophyll stability under drought stress. The transgenics also showed reduced chlorophyll retardation under NaCl, PEG and etherel-induced stress conditions. Transgenic plants showed increased yield than wild type under stress conditions. Results suggested that PG47 RNA helicase contributing for enhanced droughtadaptive traits and improved productivity under water-limited conditions. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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