| Autor: |
Mahood, Huda E., Alwash, Bushra M., Ibrahim, adhim M. |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; 2018 Supplement, Vol. 18, p913-919, 7p |
| Abstrakt: |
Moringa oleifera has been utilized as a source for bioactive compounds. Thus, this study aimed to increase alkaloids yield in callus cultures supplied with Phenylalanine (Phe) as a precursor. Seeds of M. oleifera were surface sterilized and cultured in vitro on Murashige and Skoog (MS) medium then used as a source of explants. Explants (leaves and stems) were cultured on MS medium supplemented with combinations of Benzyl adenine purine (BAP) andIndole -3- butyric acid (IBA). The best fresh weight for callus formation was achieved at1.0 mg.L-1 BAP and 1.5 mg.L-1 IBA. Quantitative analyses with High-performance liquid chromatography (HPLC) showed alkaloids presence such as Niazirin, Benzylcarbamate and Vincosamideat low levels in leaves of mother plant, (7, 9.6, 11.1 ppm respectively). The addition of different concentrations of Phe as a precursor to the mediumled to increase the levels of alkaloids in callus tissue. Phe atlow concentration (3.0 mg.L-1) enhanced production of Niazirin to 1000%, Benzylcarbamate 566.6% and Vincosamide 440.5% in comparison with mother plant. In contrast, the highest concentration of Phe (10.0 mg.L-1) was found to be inhibitory for alkaloids yield in comparison with control and other concentrations. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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