Anidulafungin increases the antibacterial activity of tigecycline in polymicrobial Candida albicans/Staphylococcus aureus biofilms on intraperitoneally implanted foreign bodies.

Autor:	Rogiers, Ona, Holtappels, Michelle, Siala, Wafi, Lamkanfi, Mohamed, Bambeke, Françoise Van, Lagrou, Katrien, Dijck, Patrick Van, Kucharíková, Soňa, Van Bambeke, Françoise, Van Dijck, Patrick, Kucharíková, Sona
Předmět:	ANTIBACTERIAL agents ECHINOCANDINS STAPHYLOCOCCUS aureus CANDIDA albicans BIOFILMS ANTIBIOTICS FOREIGN bodies PREVENTION THERAPEUTICS ANIMAL experimentation ANTIFUNGAL agents BIOLOGICAL models CANDIDIASIS COMPARATIVE studies DRUG synergism FLOW cytometry RESEARCH methodology MEDICAL cooperation MICE MICROBIAL sensitivity tests PERITONITIS RESEARCH SCANNING electron microscopy STAPHYLOCOCCAL diseases EVALUATION research TREATMENT effectiveness MIXED infections DISEASE complications PHARMACODYNAMICS
Zdroj:	Journal of Antimicrobial Chemotherapy (JAC); Oct2018, Vol. 73 Issue 10, p2806-2814, 9p
Abstrakt:	Objectives: We aimed to establish a novel murine intra-abdominal foreign body infection model to study the activity of anidulafungin and tigecycline against dual species Candida albicans/Staphylococcus aureus biofilms.Methods: In vitro and in vivo single and dual species biofilms were developed inside serum-coated triple-lumen catheters placed in 24-well plates or implanted intraperitoneally in BALB/c mice. The effect of tigecycline and anidulafungin alone and in combination was tested using clinically relevant concentrations. Scanning electron microscopy was used to visualize the mature biofilm structure developed intraperitoneally. Flow cytometry was used to determine the immunological response upon infection. Immunoblot analysis allowed us to determine the effect of anidulafungin on poly-β-(1,6)-N-acetylglucosamine in in vitro-grown S. aureus biofilms.Results: We determined the MIC, MBC and in vitro susceptibility profile for anidulafungin and tigecycline against C. albicans and S. aureus in mixed and single species biofilms. We demonstrated that anidulafungin acts synergistically when combined with tigecycline against in vivo intra-abdominal biofilms. Moreover, we reveal that anidulafungin reduces the abundance of S. aureus poly-β-(1,6)-N-acetylglucosamine. The influx of neutrophils is much increased when infected with mixed biofilms compared with single species biofilms.Conclusions: Currently, treatment of intra-abdominal infections, in particular polymicrobial catheter-associated peritonitis, is ineffective. To the best of our knowledge, this is the first study that provides insight into new possible options for treatment of C. albicans/S. aureus biofilms present in the abdominal cavity. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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