Validation of 111In-Exendin SPECT for the Determination of the β-Cell Mass in BioBreeding Diabetes-Prone Rats.
| Autor: | Brom, Maarten, Joosten, Lieke, Frielink, Cathelijne, Peeters, Hanneke, Bos, Desir'e, Boerman, Otto, Gotthardt, Martin, Zanten, Monica van, Bos, Desirée, van Zanten, Monica |
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| Předmět: |
PANCREATIC beta cells
TYPE 1 diabetes DISEASE progression DIETHYLENETRIAMINEPENTAACETIC acid RADIONUCLIDE imaging SINGLE-photon emission computed tomography PEPTIDE analysis ANIMAL experimentation COMPARATIVE studies ISLANDS of Langerhans RESEARCH methodology MEDICAL cooperation RADIOISOTOPES RATS RESEARCH EVALUATION research |
| Zdroj: | Diabetes; Oct2018, Vol. 67 Issue 10, p2012-2018, 7p |
| Abstrakt: | The changes in β-cell mass (BCM) during the development and progression of diabetes could potentially be measured by radionuclide imaging using radiolabeled exendin. In this study, we investigated the potential of 111In-diethylenetriaminepentaacetic acid-exendin-3 (111In-exendin) in a rat model that closely mimics the development of type 1 diabetes (T1D) in humans: BioBreeding diabetes-prone (BBDP) rats. BBDP rats of 4-18 weeks of age were injected intravenously with 111In-exendin, and single-photon emission computed tomography (SPECT) images were acquired. The accumulation of the radiotracer was measured as well as the BCM and grade of insulitis by histology. 111In-exendin accumulated specifically in the islets, resulting in a linear correlation with the BCM (%) (Pearson r = 0.89, P < 0.0001, and r = 0.64 for SPECT). Insulitis did not have an influence on this correlation. These results indicate that 111In-exendin is a promising tracer to determine the BCM during the development of T1D, irrespective of the degree of insulitis. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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