| Autor: |
Avşar, Timuçin, Yiğit, Berfu Nur, Turan, Gizem, Altunsu, Deniz, Mazı, Fatma Aybüke, Sürsal, Alihan, Kurt, Bahar, Bulut, İpek, Kılıç6, Türker, Durdağı, Serdar, Acar, Melih |
| Předmět: |
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| Zdroj: |
Anatomy: International Journal of Experimental & Clinical Anatomy; May2018, Vol. 12 Issue Supp1, pS93-S93, 1/2p |
| Abstrakt: |
Objective: Small molecule structured agents provide important and controllable approaches for treatment of central nervous system tumors. Their capability to easily penetrate to CNS, provide preferred therapy approach to CNS tumors. Study aims to demonstrate potential of imidazolone based small molecule in chemotherapy which led cancer cells to undergo apoptosis by stopping them in cell division. Methods: Cytotoxic effect of different structured agents, were analyzed at different concentrations. 19D's "cell cycle arrest" potential were proven at different concentrations to show characteristic of drug which specifically arrest cells. Flow cytometric analysis of cell cycle with propidium iodide was performed to six different cell lines; HUVEC, MCF-7, HCT, HEK, RFL6 and U87-MG. DNA histograms of each cell lines were figured at below and above active concentration of 19D molecule for 24 and 48-hour treatments. Alternations of cellular and nuclear morphologies were visualized via hematoxylin-eosin staining and DAPI immunofluorescence imaging. Cell proliferation indexes and viabilities of agent induced cells were analyzed with MTT assay. Results: Flow cytometric results show that agent cause G2+M arrest in all cell lines and results no further progress in cell division. Immunohistochemical and immunofluorescence staining shows that nuclear disintegration morphologies; agent cause multi lobed nucleus and multi-nucleus formations. MTT assay also provide important finding; agent activate possible apoptosis pathway in a manner of cells' division time. This finding suggest that, fast dividing cells get arrested earlier and stop their division, on the other hand slow dividing cells were arrested afterwards. Conclusion: There are many G2+M arrest causing agents currently using in chemotherapy. Their action mechanism is achieved through specifically stopping proliferation of frequently dividing cells. In this study, a new imidazolone based agent is found which specifically arrest cells and have great potential in chemotherapy. For further studies, we aim to find out specifically which molecules play role to cause this significant phenomenon in cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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