| Autor: |
Uysal, Nazan, Ateş, Mehmet, Kızıldağ, Servet, Çelik, Yasemin Seval, Kandiş, Sevim, Bozan, Hemdem Rodi, Koç, Başar, Güvendi, Güven, Karakılıç, Aslı, Çamsarı, Gamze B., İnan, Sevinç |
| Předmět: |
|
| Zdroj: |
Anatomy: International Journal of Experimental & Clinical Anatomy; May2018, Vol. 12 Issue Supp1, pS84-S84, 1/2p |
| Abstrakt: |
Objective: The aim of this study was to investigate the effects of different doses of acetaminophen on prefrontal cortex and hippocampal IGF-1 (insulin-like growht factor-1) and MMP2 (matrix metallopeptidase-2) levels, these are important for tissue integrity and health. Methods: Animals: Twenty-eight outbred male Sprague Dawley rats. All rats were divided into four groups: Control group (n=7), 100 mg/kg acetaminophen group (n=7), 200 mg/kg acetaminophen group (n=7), 400 mg/kg acetaminophen group (n=7). Biochemical analysis: The tissue homogenates were analyzed by enzyme immunoassay for IGF-1 with assay sensitivity <5 pg/mL and detection range 62.5-4000 pg/mL, MMP2 with assay sensitivity <10 pg/mL and detection range 156-10000 pg/mL. Protein analysis performed manufacturers description of BCA protein Assay kit. Histological investigation: Brain tissue samples were removed and were fixed in 10 % formaldehyde solution for 24-48 hours at room temperature. After fixation, the samples were washed overnight with tap water, dehydrated through a graded series of ethanol, cleared in xylene and further processed and embedded in paraffin. Five μm thick sections were cut using a microtome and stained with haematoxylin and eosin (H&E) and examined under a light microscope. Statistical evaluation: All statistical procedures were performed by SPSS software for Windows, Version 11.0. Results: Only 400 mg/kg dose of acetaminophen decreased IGF-1 levels in the hippocampus, whereas prefrontal cortex IGF-1 levels did not change. MMP2 levels decreased in hippocampus significantly in 400 mg/kg group. In prefrontal cortex, MMP-2 level decreased in only 400 mg/kg group with compared to 100 mg/kg group. Histologic organisational changes were observed in only hippocampus in the 400 mg/kg acetaminophen group when compared to controls. Prefrontal cortex's histological morphology was found normal. Conclusion: To our knowledge, this is the first study, which investigated effect of dose depending effects of acetaminophen on prefrontal cortex and hippocampal IGF-1 and MMP2 levels, which are related with tissue integrity and health. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
