| Autor: |
Özdemir, Özkan, Yücesan, Emrah, Kesim, F. Yeşim, Akman, Özlem, Salman, Barış, İşeri, Sibel Uğur, Özbek, Uğur |
| Předmět: |
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| Zdroj: |
Anatomy: International Journal of Experimental & Clinical Anatomy; May2018, Vol. 12 Issue Supp1, pS38-S38, 1/3p |
| Abstrakt: |
Objective: In this study, it was aimed to investigate the timedependent genomic RNA profiling of hippocampal tissues induced by febrile seizures (FS) and the epilopterogenesis process with subsequent bioinformatics approaches. Epileptogenesis is the process of developing epilepsy over normal brain differentiation in a period of time and is characterized by spontaneous seizures that develop after the initial seizure and after the seizurefree latent period. Extended febrile seizures are one of the most common causes of limbic system epilepsies at the end of epileptogenesis process. Methods: A total of 120 Sprague-Dawley rats were used in the study, consisting of 40 control and 60 FS groups divided into 12 time groups for 120 days after FS, and febrile seizure induction was performed with a vertical hot air flow model on postnatal day 10. The rats were disrupted during the possible epileptogenesis process and hippocampal material was collected. Expression profiling for a total of 28.407 transcripts was performed using Affymetrix RatGeneST2.0 microarray chip with RNA isolated from hippocampus tissues. Results: Klotho (Kl) and Transthyretin (Ttr) genes showed significant differences in FN-induced animals when the expression data set was time-dependent and multidimensional analyzed. In this context, it has been concluded that these genes are potential biomarkers for the epileptogenesis process. However, the hippocampal expression of the calcium receptor-associated olfactory receptor genes in the cell differed in animals that had febrile seizures. Conclusion: Kl and Ttr genes were found to be important roles in latent period after febrile seizure and they were selected as candidates for meta-analysis studies. However, the difference in olfactory receptor gene expression has led to the interpretation that long-term accumulation of intracellular calcium in the epileptogenesis process may be regulated by these genes. Findings are very important in terms of understanding the nature of epilepsy and febrile seizures. This study was supported by the project numbered 214S222 of TUBITAK. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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