| Abstrakt: |
In this study, proteases have been isolated from agricultural soil samples and then cultured by shake flask method. The growth of the Bacillus subtilis has been confirmed by microbiological test on the agar plate and skim milk agar in rough, raised and irregular colonies. The yield of the alkaline protease has been optimised by varying the main factors i.e., nitrogen source (peptone, yeast extract, beef extract, casein, ammonium carbonate and urea), carbon source (sucrose, fructose, mannose, lactose, glucose, maltose and starch), incubation period (12, 24, 36, 48, 72, 84 and 96 h), temperature (35, 40, 45, 50, 55, and 60 °C) and salts (potassium sulphate, magnesium sulphate, calcium sulphate and manganese sulphate). The results revealed that the maximum enzyme production was obtained using casein and minimum activity was obtained using urea as a nitrogen source. Similarly, other factors have shown significant effect on the activity of the enzyme. [ABSTRACT FROM AUTHOR] |
