| Autor: |
Santoro, Lyse, Reboul, Angeline, Kerblat, Isabelle, Drouet, Christian, Colomb, Maurice G. |
| Zdroj: |
International Immunology; Feb1996, Vol. 8 Issue 2, p211-219, 9p, 6 Graphs |
| Abstrakt: |
Murine mAb injected into patients behave as exogenous antigens and trigger a specific immune response characterized mainly by CD4+ T lymphocytes. They are recognized by T cells under a processed form and in a MHC class II-restricted fashion. IgG degradation which occurs in antigen-presenting cells (APC) has been studied in vitro. We have shown that partial reduction of this antigen is an early event which is significant for the generation of class ll-restricted fragments presentable to antigen-specific T cells. Two different murine mAb were used as antigens and human monocytic U937 or antigen non-specific Epstein-Barr virus-transformed B cells as APC. Upon cellular internalization IgG are rapidly cleaved leading to 24–25 kDa fragments. One of the major and early events corresponds to partial reduction of IgG—the light chain is released from the intact molecule, heavy chains remaining bound together. Partialin vitro reduction of IgG followed by presentation by fixed B cells to specific T cells showed that onlyk light chain-specific T cell clones are stimulated, in contrast to heavy chain-specific T cell clones. The response to the k chains of specific T cells points to a significant role played by the early IgG partial reduction in the generation of k light chain class II binding fragments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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