| Autor: |
Ramesha, C. B., Rathnamma, D., Isloor, S., Veeregowda, B. M., Narayanaswamy, H. D., Narayana, M. Narayana, Siddalingamurthy, H. K., Ganesh, K., Mukartal, S. Y., Sreesujatha, R. M., Pannaga, P. |
| Předmět: |
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| Zdroj: |
Journal of Experimental Zoology India; Jul2018, Vol. 21 Issue 2, p885-892, 8p |
| Abstrakt: |
The present study was conducted to evaluate efficacy of an indigenous inactivated MAP vaccine in experimentally challenged sheep. S196 MAP isolate of sheep origin was inactivated at 720 C for 2 hours and vaccine was prepared at a final concentration of 2.5 mg (dried) of MAP organisms per ml (approximately 12×108 bacilli/ml) using Aluminum hydrooxide gel and 0.01% Thiomersal. Experimental animals of 4-6 months of age were grouped into vaccinated and sham immunized animals which were vaccinated with inactivated MAP vaccine and challenged at 75th, 77th & 79th DPV with 3×109 CFU of live MAP organisms through oral route. One animal from each group were sacrificed at 90th, 240th and 300th DPV. Animals were evaluated for clinical symptoms, cell mediated and humoral immune responses, shedding of MAP bacilli and pathology. Vaccinated sheep gained higher body weight, also had significant lymphocyte stimulation and higher sero-conversion as compared to sham immunized sheep. There was significant difference between (P<0.05) vaccinated and sham immunized animals in all immunological parameters. The shedding of MAP bacilli was observed in sham immunized animals after 150 days post challenge. Euthanized sham immunized animals showed pathognomonic gross lesions of JD, which were not observed in vaccinated animals. In conclusion, indigenous inactivated S196 MAP vaccine had protected the sheep against challenge infection with live MAP organisms with an effective CMI and humoral immune responses. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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