| Abstrakt: |
Abstract: Cations‐induced DNA aggregation can modify the local structure of oligonucleotides and has potential applications in medicine and biotechnology. Here, we used atomic force microscopy to investigate λ‐DNA aggregation on Mg2+‐treated glass (Mg2+/glass) and in Mg2+ solution. Atomic force microscopy topography images showed that some DNA fragments were slightly stacked together on 10 mM Mg2+/glass and stacked stronger on ≥50 mM Mg2+/glass. They also showed that DNA aggregated stronger in Mg2+ solution than on Mg2+/glass, ie, DNAs are strongly stacked and twisted at 10 mM Mg2+, rolled together at 50 mM Mg2+, and slightly aggregated to form small particles at 100 mM Mg2+. At a specific condition, ie, heating λ‐DNA to 92°C, cooling down to 75°C, adding Mg2+, and vortexing the resulting solution, DNA strongly aggregated and formed pancake‐like shapes at 10 and 50 mM or a large aggregate at 100 mM Mg2+ solutions. Our results may be helpful for medical applications and gene therapy using cation‐DNA technology. [ABSTRACT FROM AUTHOR] |
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