| Autor: |
Karimi, Hesam, Soleimanjahi, Hoorieh, Abdoli, Asghar, Seyed Mahmood Seyed Khorrami, Shirmohammadi, Masoumeh, Banijamali, Razieh Sadat |
| Předmět: |
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| Zdroj: |
Iranian Journal of Allergy, Asthma & Immunology; 2018 Supplement, Vol. 17, p230-231, 2p |
| Abstrakt: |
Objective: A main goal of modern vaccinology is to emulate the efficacy of such live vaccines with suitable adjuvant end, defined acellular vaccines. Therefore, the success of cancer vaccines depends on identification of specific antigenic targets and the ability to evoke a strong and appropriate immune response. Nanoparticulate carriers provide adjuvant activity by enhancing antigen delivery or by activating innate immune responses. The nanovesicles such as archaeosomes have become important carrier systems. Archaeosomes are prepared from archaeal glycerolipids which show great adjuvant activity and capability to promote both Th1 and Th2 response with long memories and can be used in drug, gene and vaccine delivery. Among therapeutic HPV vaccines, DNA vaccines have emerged as a potentially promising approach due to their safety profile, simplicity of preparation and stability. In this study, archaeosomes are prepared from polar lipid of the Halobacterium salinarum and the formulation of the E6/E7/L1 chimeric gene with archaeosome as a Human papillomavirus (HPV) vaccine candidate and examined in tumor mice model. Material and Methods: The recombinant pIRES2- E6/E7/L1 chimeric plasmid of HPV were multiplied in a DH5α strain of Escherichia coli and purified using a modification of alkaline lysis maxi-preparation. Archaeosomes prepared with total polar lipids of Halobacterium salinarum by the method of Bligh and Dyer. The formation of the archaeosome-pDNA complex was achieved by the addition of plasmid DNA to archaeal lipid solution and the mixture was kept at room temperature for several hours to allow complex formation to take place. Particle sizes and zeta potential of the samples was measured using dynamic light scattering [Zetasizer Nano ZS (Malvern Instruments)]. After development of tumor by administration of TC-1 cells in C57BL/6 mice, relative tumor volume measurements were carried out. Results: Results showed that Archaeosome containing E6/E7/L1 chimeric gene significantly inhibit the rate of tumor growth in comparison with control groups. Conclusion: In general, due to the suitable inhibitory effect of archaeosome-pDNA complex in reduction of tumor size, they can be considered as a good therapeutic strategy and may be a promising candidate for development of therapeutic vaccine against HPV-16 cancers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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