| Autor: |
Li, Anzhong, Yu, Jingyou, Lu, Mijia, Ma, Yuanmei, Attia, Zayed, Shan, Chao, Xue, Miaoge, Liang, Xueya, Craig, Kelsey, Makadiya, Nirajkumar, He, Jennifer J., Jennings, Ryan, Shi, Pei-Yong, Peeples, Mark E., Liu, Shan-Lu, Boyaka, Prosper N., Li, Jianrong |
| Zdroj: |
Nature Communications; 8/3/2018, Vol. 9 Issue 1, p1-1, 1p |
| Abstrakt: |
Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response and protection is poorly understood. Here, we develop an attenuated recombinant vesicular stomatitis virus (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored vaccine candidate is attenuated in mice, where a single immunization induces ZIKV-specific antibody and T cell immune responses that provide protection against ZIKV challenge. Co-expression of prM, E, and NS1 induces significantly higher levels of Th2 and Th17 cytokine responses than prM-E. In addition, NS1 alone is capable of conferring partial protection against ZIKV infection in mice even though it does not induce neutralizing antibodies. These results demonstrate that attenuated rVSV co-expressing prM, E, and NS1 is a promising vaccine candidate for protection against ZIKV infection and highlights an important role for NS1 in ZIKV-specific cellular immune responses. Current Zika virus (ZIKV) subunit vaccine development largely focuses on prM and E proteins, and the role of NS1 for immune response and protection is unclear. Here, Li et al. develop an attenuated VSV-based vaccine expressing a ZIKV prM-E-NS1 polyprotein and characterize immune response and protection in mice. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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