| Autor: |
Engel, Thomas, Lueken, Aloys, Bode, Günther, Hobohm, Uwe, Lorkowski, Stefan, Schlueter, Bernhard, Rust, Stephan, Cullen, Paul, Pech, Michael, Assmann, Gerd, Seedorf, Udo |
| Předmět: |
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| Zdroj: |
FEBS Letters; May2004, Vol. 566 Issue 1-3, p241-246, 6p |
| Abstrakt: |
Here, we identify ADP-ribosylation factor (ARF)-like 7 (ARL7) as the only ARF- and ARL-family member whose mRNA-expression is induced by liver X-receptor/retinoid X-receptor agonists or cholesterol loading in human macrophages. Moreover, subcellular distribution of mutant and wild type ARL7-enhanced green fluorescent protein (EGFP) supports that ARL7 may be involved in a vesicular transport step between a perinuclear compartment and the plasma membrane. Therefore, we investigated the effect of ARL7 over-expression on the cholesterol secretory pathway. We found that expression of wild type and dominant active ARL7-EGFP stimulated the rate of apolipoprotein AI-specific cholesterol efflux 1.7- and 2.8-fold. In contrast, expression of the dominant negative form of ARL7-EGFP led to ∼50% inhibition of cholesterol efflux. This data is consistent with a model in which ARL7 is involved in transport between a perinuclear compartment and the plasma membrane apparently linked to the ABCA1-mediated cholesterol secretion pathway. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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