| Autor: |
Hawkesford, Malcolm J., Reed, Robert H., Rowell, Peter, Stewart, William D. P. |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 4/15/81, Vol. 115 Issue 3, p519-523, 5p |
| Abstrakt: |
Relationships between nitrogenase activity and individual components of the proton electrochemical potential gradient (ΔμH) in Anabaena variabilis have been investigated. The ionophore nigericin was found to collapse ΔpH-in favour of the membrane potential (Δψ); hyperpolarization of Δψ was correlated with an increase in nitrogenase activity. A positive relationship between nitrogenase activity and membrane potential was also observed using the ionophore valinomycin and the uncoupler carbonylcyanide m-chlorophenylhydrazone. Furthermore, using the energy transfer inhibitor N,N′-dicyclohexylcarbodiimide, nitrogenase activity appeared to be limited by the supply of reductant rather than ATP. The data suggest, for intact filaments and isolated heterocysts, that Δψ may be important in regulating nitrogenase activity in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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