| Autor: |
Ferreira-Silva, José Carlos, Motta Rocha, Jorge, Lima Basto, Sarah Romini, Nunes Ferreira, Heder, Melo Souza, Helder, Freitas Neto, Leopoldo Mayer, Moura, Marcelo Tigre, Cavalcanti Oliveira, Marcia Cristina, Lemos Oliveira, Marcos Antonio |
| Předmět: |
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| Zdroj: |
Pferdeheilkunde; jan/feb2018, Vol. 34 Issue 1, p51-56, 6p |
| Abstrakt: |
The aim was to verify the efficiency of different glycerol concentrations (1%, 3%, and 5%) for cryopreservation of stallion semen. After semen collection, sperm cells were evaluated for motility and acrosin activity. The semen was divided into three equal parts and resuspended in freezing diluent without glycerol in a 1:1 proportion and were incubated for 20 minutes at room temperature. Immediately after centrifugation for 10 minutes at 600g and discarding the supernatant, each pellet was diluted with the same diluent and further centrifuged for 10 minutes. After the second centrifugation, each pellet received freezing glycerol-containing diluent under concentrations of 1%, 3%, and 5% and was loaded in 4mL macro-tubes. These macro-tubes were placed in liquid nitrogen vapor for 15 minutes for immediate immersion in liquid nitrogen and storage at -196°C. After thawing at 50°C for 40 seconds, semen samples were initially evaluated for sperm motility and acrosin activity. Moreover, semen was also incubated at 37ºC and evaluated for sperm motility after 60 and 120 minutes in a thermoresistance test (TRT). Immediately after thawing and during the TRT, both progressive and total sperm motility was lower in 1% glycerol (P0.05) between these two later concentrations. The acrosin activity in fresh semen was only greater (P0.05) between these later glycerol concentrations. In conclusion, based on sperm motility and acrosin activity, that 3% and 5% glycerol concentrations are suitable for stallion semen cryopreservation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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