Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts.

Autor:	Modéer, T., Domelj, H., Andurén, Mustafa, M., Brunius, G., Modéer, T, Domeij, H, Andurén, I
Předmět:	PHENYTOIN INTERLEUKIN-6 INTERLEUKIN-8 FIBROBLASTS GENETIC transcription MESSENGER RNA RNA analysis ANTICONVULSANTS BIOCHEMISTRY CELL culture COMPARATIVE studies DRUG synergism ENZYME-linked immunosorbent assay GINGIVA IN situ hybridization INTERLEUKIN-1 INTERLEUKINS PHENOMENOLOGY RESEARCH methodology MEDICAL cooperation PHARMACOLOGY RESEARCH EVALUATION research PHARMACODYNAMICS
Zdroj:	Journal of Oral Pathology & Medicine; Nov2000, Vol. 29 Issue 10, p491-499, 9p
Abstrakt:	The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1beta (IL-1beta), was studied. PHT (20 microg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1beta (30 pg/ml). The stimulatory effect of PHT on IL-1beta-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 microM). The anti-inflammatory drug, dexamethasone (1 microM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1beta. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Plný text