| Autor: |
Skrukrud, C. L., Taylor, S. E., Hawkins, D. R., Nemethy, E. K., Calvin, M. |
| Předmět: |
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| Zdroj: |
Physiologia Plantarum; Oct88, Vol. 74 Issue 2, p306-316, 11p |
| Abstrakt: |
Latex isolated from laticifer cells of Euphorbia lathyris maintained its ability to synthesize triterpenols (and their esters) from acetate. When the latex was centrifugated at 5000 g for 15 mm, this biosynthetic activity could be subdivided into two separate fractions: the acetate to β-hydroxymethyl glutaryl-coenzyme A activity remained in the supernatant, while the β-hydroxymethyl glutaryl-coenzyme A to triterpenol activity was pelleted. Further purification of the pellet by isopycnic centrifugation on Percoll gradients yielded at least three particles: latex particles, starch grains, and a single membrane-bound organelle. Electron micrographs were made of all of these latex particles. The single membrane-bound organelle was only observed in the region of the density gradient that exhibited the ability to incorporate mevalonic acid into the triterpenoids. In addition, the enzyme β-hydroxymethyl glutaryl-coenzyme A reductase (EC 1.1.1.34) was found in the 5000 g pellet, while β-hydroxymethyl glutaryl-coenzyme A lyase (EC 4.1.3.4) remained in the super- natant. |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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