MicroRNA-218 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells and Accelerates Bone Fracture Healing.

Autor:	Shi, Liu, Feng, Lu, Liu, Yang, Duan, Ji-qiang, Lin, Wei-ping, Zhang, Jin-fang, Li, Gang
Předmět:	MICRORNA CELL differentiation BONE growth BONE fractures MESENCHYMAL stem cells WOUND healing GENETICS RNA physiology ANIMAL experimentation BONE marrow BONES CELL culture CELL physiology COMPUTED tomography FEMUR injuries IMMUNOHISTOCHEMISTRY MICE RESEARCH funding PHYSIOLOGIC strain FRACTURE healing
Zdroj:	Calcified Tissue International; Aug2018, Vol. 103 Issue 2, p227-236, 10p
Abstrakt:	As a regulator of osteogenesis, microRNA-218 (miR-218) is reported to promote osteogenesis of mesenchymal stem cells (MSCs). However, the in vivo osteogenic effect of miR-218 remains elusive. In this study, miR-218 was confirmed to promote osteogenic differentiation of MSCs by stimulating the alkaline phosphatase activity, calcium nodule formation, and osteogenic marker gene expression. For in vivo study, the miR-218-overexpressing BMSCs were locally administrated into the fracture sites in a femur fracture mouse model. Based on the X-rays, micro-computed tomography, mechanical testing, histology, and immunohistochemistry examinations, miR-218 overexpression improved new bone formation and accelerated fracture healing. These findings suggest that miR-218 may be a promising therapeutic target for bone repair in future clinical applications. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
Externí odkaz:	Zobrazit plný text záznamu Full text from SpringerLink