α-Tocopherol influences glycaemic control and miR-9-3 DNA methylation in overweight and obese women under an energy-restricted diet: a randomized, double-blind, exploratory, controlled clinical trial.

Autor: Luna, Rafaella Cristhine Pordeus, dos Santos Nunes, Mayara Karla, Monteiro, Mussara Gomes Cavalcante Alves, da Silva, Cássia Surama Oliveira, do Nascimento, Rayner Anderson Ferreira, Lima, Raquel Patrícia Ataíde, Pimenta, Flávia Cristina Fernandes, de Oliveira, Naila Francis Paulo, Persuhn, Darlene Camati, de Almeida, Aléssio Tony Cavalcanti, da Silva Diniz, Alcides, Pissetti, Cristina Wide, Vianna, Rodrigo Pinheiro Toledo, de Lima Ferreira, Flavia Emília Leite, Rodrigues Gonçalves, Maria da Conceição, de Carvalho Costa, Maria José
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Zdroj: Nutrition & Metabolism; 7/11/2018, Vol. 15 Issue 1, pN.PAG-N.PAG, 1p, 1 Diagram, 2 Charts, 2 Graphs
Abstrakt: Background: Excess weight is a strong risk factor for the development of dysglycaemia. It has been suggested that changes in the metabolism microRNAs, small non-coding RNAs that regulate gene expression, could precede late glycaemic changes. Vitamin E in turn may exert important functions in methylation and gene expression processes. This study aimed to determine the effect of α-tocopherol on glycaemic variables and miR-9-1 and miR-9-3 promoter DNA methylation in overweight women. Methods: A randomized, double-blind, exploratory, placebo-controlled study was conducted in overweight and obese adult women (n = 44) who ingested synthetic vitamin E (all-rac-α-tocopherol), natural source vitamin E (RRR-rac-α-tocopherol) or placebo capsules and were followed up for a period of 8 weeks. Supplemented groups also received dietary guidance for an energy-restricted diet. An additional group that received no supplementation and did not follow an energy-restricted diet was also followed up. The intervention effect was evaluated by DNA methylation levels (quantitative real-time PCR assay) and anthropometric and biochemical variables (fasting plasma glucose, haemoglobin A1C, insulin, and vitamin E). Results: Increased methylation levels of the miR-9-3 promoter region (P < 0.001) and reduced haemoglobin A1C (P < 0.05) were observed in the natural source vitamin E group after intervention. Increased fasting plasma glucose was observed in the synthetic vitamin E group, despite the significant reduction of anthropometric variables compared to the other groups. Conclusions: α-Tocopherol from natural sources increased methylation levels of the miR-9-3 promoter region and reduced haemoglobin A1C in overweight women following an energy-restricted diet. These results provide novel information about the influence of vitamin E on DNA methylation. Trial registration: ClinicalTrials.gov, NCT02922491. Registered 4 October, 2016. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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