| Autor: |
Hwang, Cheol-Hwan, Park, Hae-Min, Park, Han-Gyu, Ahn, Da-Hee, Kim, Seong-Min, Ko, Byoung Joon, Kim, Young Hwan, Yang, Yung-Hun, Kim, Yun-Gon |
| Zdroj: |
Korean Journal of Chemical Engineering; Jul2018, Vol. 35 Issue 7, p1462-1467, 6p |
| Abstrakt: |
For validating O-glycosylation of protein biotherapeutics, we presented a quantitative O-glycomics method which is based on the neutralization of sialic acids, the specific release of O-glycans, and the introduction of permanent positive charge followed by quantitative MALDI-MS analysis. This method shows excellent technical reproducibility, linearity and sensitivity. In addition, it enables the quantification of intact O-glycans with minimal degradation or loss of sialic acids on these glycans compared to a conventional HPLC-based method. We then applied this method to quantitatively characterize O-glycans present on Etanercept. The analysis showed the relative abundances of mono- and di-sialylated core 1 O-glycans - were 79.3±0.8% and 17.3±1.4%, respectively. This glycomics technology could allow for the reliable quantitative analysis of intact O-glycans from glycoproteins and may contribute to validation of O-glycosylation protein biotherapeutics in the pharmaceutical industry. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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