Expression of the E-selectin gene in human gingival epithelial tissue.

Autor:	Pietrzak, Eva R., Savage, Nell W., Aldred, Michael J., Walsh, Laurence J., Pietrzak, E R, Savage, N W, Aldred, M J, Walsh, L J
Předmět:	CELL adhesion molecules SELECTINS GINGIVAL hyperplasia EPITHELIAL cells CYTOKINES KERATINOCYTES RNA analysis ANTIGENS CELL culture COMPARATIVE studies DOCUMENTATION EPITHELIUM GENE expression GINGIVA RESEARCH methodology MEDICAL cooperation NUCLEOTIDES ORAL mucosa RESEARCH EVALUATION research
Zdroj:	Journal of Oral Pathology & Medicine; Jul1996, Vol. 25 Issue 6, p320-324, 5p
Abstrakt:	E-selectin is an adhesion molecule, expressed by cytokine-activated endothelial cells, that participates in the binding of neutrophils. Recent studies in our laboratory documented binding of the E-selectin-specific monoclonal antibody H4/18 to keratinocytes in inflamed human oral mucosa, particularly gingival epithelium. To determine whether this immunoreactivity was due to expression of authentic E-selectin, the presence of E-selectin mRNA in gingival epithelium was analysed using the polymerase chain reaction (PCR). Reverse transcription of epithelial RNA and amplification of cDNA with E-selectin-specific primers resulted in the formation of a 178 nucleotide PCR product identical to that obtained from cytokine-activated endothelial cells. Sequencing of the PCR product revealed 100% homology between epithelial and endothelial E-selectin fragments. Epithelial preparations did not contain mRNA for von Willebrand factor, excluding the possibility of contamination by endothelial cells. These results confirm immunohistochemical studies of E-selectin immunoreactivity in human oral mucosa and demonstrate that E-selectin expression is not confined to endothelium. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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