| Autor: |
Morabito, Rossana, Remigante, Alessia, Arcuri, Benedetta, Marino, Angela, Giammanco, Marco, Spada, Giuseppina La |
| Předmět: |
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| Zdroj: |
Journal of Biological Research / Bollettino della Società Italiana di Biologia Sperimentale; 2018, Vol. 91 Issue 1, p1-7, 7p |
| Abstrakt: |
The efficiency of transport through Band 3 protein, mediating HCO3-/Cl- exchange across erythrocytes membrane, is reduced by oxidative stress. The aim of the present study was to verify whether Band 3 protein transport efficiency is compromised by treatment with Cadmium (Cd2+), an extremely toxic heavy metal known to interfere with antioxidant enzymes, energy metabolism, gene expression and cell membranes. To this end, the rate constant for SO4= uptake through Band 3 protein (accounting for velocity of anion exchange) was measured along with membrane -SH groups, Malonyldialdehyde (MDA) and Band 3 protein expression levels in Cd2+-treated human erythrocytes (300 μM, 1 mM). Our results show that Cd2+ reduced the rate constant for SO4 = uptake, with a significant increase in MDA levels at both concentrations and with a reduction in -SH groups observed after 1 mM Cd2+ treatment, whereas Band 3 protein expression levels were unchanged in both conditions. In conclusion: i) Cd2+ reduces Band 3 protein transport efficiency via different mechanisms depending on metal concentration and with unchanged expression levels; ii) the assessment of Band 3 protein anion exchange capability is a good tool to assay the impact of heavy metals on cell homeostasis and, possibly, useful for diagnosis and monitoring of development of Cd2+ toxicity-related pathologies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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