| Autor: |
Rondot, Isabelle, Quennedey, Brigitte, Courrent, Annie, Lemoine, Aleth, Delachambre, Jean |
| Předmět: |
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| Zdroj: |
European Journal of Biochemistry; 1/15/96, Vol. 235 Issue 1/2, p138-143, 6p |
| Abstrakt: |
A eDNA clone encoding a larval-pupal cuticular protein, named TMLPCP-22, has been isolated by screening a library m expression vector with a monoclonal antibody made against pupal cuticular proteins of Tenebrio molitor. Northern-blot and in situ hybridization analyses showed that the expression of TMLPCP-22 is regulated in a stage-specific and tissue-specific manner; the transcript was present during the secretion of preecdysial larval and pupal cuticles and was restricted to epidermal cells, No expression wits observed during adult cuticle deposition. In supernumerary pupae obtained after application of a juvenile hormone analogue, which is known to inhibit the adult programme. TMLPCP-22 mRNA was expressed again, confirming its larval-pupal specificity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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