P2Y2R deletion ameliorates sialadenitis in IL‐14α‐transgenic mice.

Autor:	Woods, L. T., Camden, J. M., Khalafalla, M. G., Petris, M. J., Erb, L., Ambrus, Jr, J. L., Weisman, G. A.
Předmět:	GENETICS of autoimmune diseases AUTOIMMUNE disease prevention SALIVA analysis SIALADENITIS ANIMAL experimentation B cells CELL receptors GENE expression INTERLEUKINS MICE PARASYMPATHOMIMETIC agents SECRETION SJOGREN'S syndrome SUBMANDIBULAR gland T cells TRANSGENIC animals TUMOR necrosis factors GENETICS PREVENTION
Zdroj:	Oral Diseases; Jul2018, Vol. 24 Issue 5, p761-771, 11p, 5 Graphs
Abstrakt:	Objective: Interleukin‐14α‐transgenic (IL‐14αTG) mice develop an autoimmune exocrinopathy with characteristics similar to Sjögren's syndrome, including sialadenitis and hyposalivation. The P2Y2 receptor (P2Y2R) for extracellular ATP and UTP is upregulated during salivary gland inflammation (i.e., sialadenitis) where it regulates numerous inflammatory responses. This study investigated the role of P2Y2Rs in autoimmune sialadenitis in the IL‐14αTG mouse model of Sjögren's syndrome. Materials and Methods: IL‐14αTG mice were bred with P2Y2R−/− mice to generate IL‐14αTG × P2Y2R−/− mice. P2Y2R expression, lymphocytic focus scores, B‐ and T‐cell accumulation, and lymphotoxin‐α expression were evaluated in the submandibular glands (SMG) along with carbachol‐stimulated saliva secretion in IL‐14αTG, IL‐14αTG × P2Y2R−/−, and C57BL/6 control mice at 9 and 12 months of age. Results: Genetic ablation of P2Y2Rs in IL‐14αTG mice significantly reduced B and T lymphocyte infiltration of SMGs. However, reduced sialadenitis did not restore saliva secretion in IL‐14αTG × P2Y2R−/− mice. Decreased sialadenitis in IL‐14αTG × P2Y2R−/− mice correlated with decreased lymphotoxin‐α levels, a critical proinflammatory cytokine associated with autoimmune pathology in IL‐14αTG mice. Conclusions: The results of this study suggest that P2Y2Rs contribute to the development of salivary gland inflammation in IL‐14αTG mice and may also contribute to autoimmune sialadenitis in humans. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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